LONG AMPLICONS AS A TOOL TO IDENTIFY VARIABLE REGIONS OF RIBOSOMAL RNA FOR IMPROVED TAXONOMIC RESOLUTION AND DIAGNOSTIC ASSAY DESIGN IN MICROEUKARYOTES: USING ASCETOSPOREA AS A CASE STUDY

IntroductionThere is no universally appropriate basis for delimiting species in protists, including parasites.Many molecular markers used for species delimitation are part of the ribosomal RNA (rRNA) array, with different regions of the array being used for different parasitic protist taxa.However, little is known about sequence variability across

Abstract For places where non-sterile drug production occurs, regulatory bodies recommend monitoring of the environmental bioburden.This procedure provides information regarding possible microbiological risks to which the products may be exposed, so that subsequent action measures may be implemented.The aim of the present work was to quantify and c

Abstract Phosphodiesterase type 5 inhibitors (PDE5is) constitute the primary therapeutic option for treating erectile dysfunction (ED).Nevertheless, a substantial proportion of patients, approximately 30%, do not respond to PDE5i treatment.Therefore, new treatment methods are needed.In this study, we identified ucsb gaucho blue a pathway that contr